Sebenzile is a development consultant and has studied at the University of Botswana and Swaziland where she obtained a B. Sc. Biology, Statistics and Environmental Science. She furthered her studies at the Pennsylvania State University, State College, USA in a M. Sc Biometrics and an incomplete Ph. D in Biometrics at the University of Saskatchewan, Saskatoon in Canada. Sebenzile worked in the Office on the Status of Disabled People in the Presidency where she served as Director of the OSDP from 1997 to 2005. Thereafter she joined the private sector as Executive Chairperson of Divuseni (Pty) Ltd an investment company of women with disabilities; an Executive Director of Motswako-Ricoh, an office automation supplier and also a non-executive director of the African Access Holdings (Pty) Ltd group of companies.
Sebenzile is active internationally in the work of the United Nations and its organs; the African Union; the World Bank and the disability movement. She consults for various government departments; parastatal companies and private companies in the field of disability, human rights and development. She serves as a Trustee on the boards of Action on Disability and Development international; the South African Development Trust for Disabled People, the Centre for Alternate and Augmentative communication of the University of Pretoria, Cheshire Homes of South Africa and the FirstRand Foundation. Sebenzile previously also served on the boards of the Telkom Foundation, the African Decade of Disabled Persons and Rehabilitation International.
Sebenzile was appointed to the DEC Investment Holding Company Board in November 2005 as a non-executive director.